UV/VIS Absorbance Measurements
Absorbance measurements in fluids can be done in different setups and wavelength ranges, for instance with fibre optic dip probes, flow cells for in-line absorbance or a cuvette holder for absorption measurement in a sample.
For UV/VIS measurements, a spectrometer can be configured with a range from 200 to 1100nm and a spectral resolution of 1.4 nm FWHM. In addition, a combined deuterium-halogen light source is needed. For the different applications different probes can be used.
A typical setup for absorption measurement is shown in the illustration above.
Typical components used in UV/VIS absorbance measurements
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Inline absorption with flowcell |
Inline absorption with dip probe |
Inline absorption with cuvette holder |
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Spectrometer |
AvaSpec-2048, grating UA (200-1100nm), DUV, 25µm slit, DCL-UV, OSC-UA | ||
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Software |
AvaSoft-FULL and optionally AvaSoft-CHEM | ||
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Light source |
AvaLight-DHS-BAL | ||
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Fibre optics |
2x FC-UV200-2-SR, 200µm detection and illumination fibres, UV/VIS, solarisation resistant, 2m |
FDP-7UV200-2-VAR transmission dip probe with variable pathlength, 6x 200µm illumination fibres, 1x 200µm read fibre, UV/VIS, 2m |
2x FC-UV200-2-SR, 200µm detection and illumination fibres, UV/VIS, solarisation resistant, 2m |
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Accessories |
¼” or ½” flow cell |
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CUV-UV/VIS cuvette holder |
